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Determination of some nutrition factors in selected kinds of fruit
Smělá, Margita ; Vítová, Eva (referee) ; Vespalcová, Milena (advisor)
Diploma thesis is concerned with determination of some nutrion factors and vitamin C in various cultivars of apples (Pirus Malus, L.) and quinces (Cydonia oblonga, Mill.). In theoretical part there are described general characterization in selected kinds of fruits, their medically significant sunstances and utilization of these fruits. Main focus is paid to vitamin C. There are described its chemical properties and effect for human health. Various methods for determination of some nutrion factors with focus on determination of vitamin C by high-performance liquid chromatography. In experimental part, total of 27 various cultivars of aplles and 16 various cultivars of quinces were analyzed. These cultivars are from grower station Faculty of garden of Mendel university. For determitation vitamin C was used by high-performance liquid chromatography. Vitamin C was determination as an ascorbic acid. Some nutrion factors were analyzed in experimental part. There were analyzed dry matter, total flavonoids, total phenolics and titratable acid in various cultivars of apples and quinces. There were compared of some various cultivars after representation and content of significant substances as result. The last part of thesis was sensory analysis of apples.
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Hmyzí škůdci ovocných dřevin v sadech postižených tornádem
Mančíková, Radka
Observations of insect pests were carried out in 2022 and 2023 on domestic apple trees in two orchards in the Hodonín region, in Lužice, in an orchard affected by a tornado, and in Prušánky. During the observation of overwintering stages of pests, a total of 6 159 individuals from two orders, four families, were recorded in 2022. The most abundant family was Diaspididae (5 922 individuals), followed by Tetranychidae (151 individuals), Psyllidae (63 individuals), and Aphididae (22 individuals). In 2023, there was a significant decline in the abundance of representatives of all families. On average, the abundance of pests was higher in the experimental plot in Prušánky. Two species – Aphis pomi and Eriosoma lanigerum – were recorded during aphid observations during the growing season. A total of 733 colonies were recorded, the species distribution was roughly the same in both plots. The apple trees damaged by the tornado had the lowest number of aphid individuals. Using a pheromone trap for Cydia pomonella, its occurrence was confirmed in both experimental plots. The observation period was from 1 May to 31 August, and two peaks of occurrence were recorded during this period. With beating, total of 331 individuals, 7 insect species from 5 families, were recorded during the period from 22 August to 25 September. The most abundant species was the apple sawfly (Hoplocampa testudinea) (80 individuals).
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Mšice na ošetřovaných a neošetřovaných ovocných dřevinách
Prudíková, Tereza
During the year 2022, the occurrence of aphids on apple trees with different protection intensities was monitored at two locations and five experimental areas in Starý Lískovec (Brno) and in Květná (district Uherské Hradiště). The monitoring of overwintering eggs was realized in February. It was found, that higher abundance of eggs was on south side and lower level of the crown. The highest number of eggs was discovered on untreated apple orchard Květná, with an average of 32 eggs per 1 m of branch length. Visual control of the abundance and species spectrum of Aphididae was realized on 3 June, 29 June and 16 July 2022. Totally four aphid species were detected on three experimental areas, Dysaphis plantaginea, Myzus persicae, Aphis pomi and Eriosoma lanigerum. The highest abundance and most species were detected on untreated part in Starý Lískovec, summary 26 colonies of three different aphid species (Dysaphis plantaginea, Myzus persicae, Eriosoma lanig-erum).
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Diversity of Neofabraea species causing bull's eye rot in the Czech Republic
Pešicová, Kamila ; Novotný, David (advisor) ; Mazáková, Jana (referee)
Neofabraea is a genus of an important plant pathogenic fungi having worldwide appearance. Four Neofabraea species are responsible for bull's eye rot of pome fruits. The aim of this thesis was to investigate which of these species occur in the Czech Republic. 81 isolates were collected during a two- year period and they were identified using PCR fingerprinting (primers ERIC 1R and M13-core) and DNA sequencing (ITS, mtSSU and tub2). The results showed that species N. alba, N. perennans and Cryptosporiopsis kienholzii occur in the Czech Republic. According to available information, this is the second record of C. kienholzii in Europe. One isolate (KP4) failed to be identified as any of the species. KP4 is very close to C. kienholzii, but it can be distinguished both biologically and genetically. Furthermore, the aggressiveness of individual species was compared.N. perennans and strain KP4 proved to be most aggressive, the least aggressive is C. kienholzii. Two N. alba strains (KP36 and KP37) isolated from healthy apple fruit and leaf are pathogenic for apple fruits. Keywords: aggressiveness, Cryptosporiopsis kienholzii, Dermateaceae, Helotiales, apple tree, Malus, PCR fingerprinting, postharvest diseases Powered by TCPDF (www.tcpdf.org)
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Apple Trees as Representatives the Utility Plants of the Tribe Rosaceae
Šnorová, Markéta ; Skýbová, Jana (advisor) ; Odcházelová, Tereza (referee)
TITLE Apple trees as representatives of utility plants in the menials Rosaceae AUTHOR Markéta Šnorová DEPARTMENT Department of biology and environmental education SUPERVISOR RNDr. Jana Skýbová ABSTRACT Submitted bachelor thesis is devoted to apple trees, as represantatives of menials Rosaceae. At first I introduced some other plants of family Rosaceae. My object was process theme apple tree from wider angle of view. From pollination until salvage and use their fetuses - apples. I have dealt also ecological requirements of appletree, their cultivation, varieties and history of their cultivation. Further I have highlighted importance of apples for humans in terms of nutrition. For the purpose of teaching i have compiled practical works useful in the lessons of botany at elementary school. And grammar school I suggested and laboratories tasks of chemistry and biology. KEY WORDS Apple tree, apple, cultivation, variety, nutrition.
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Apple dormant bud cryopreservation: Methodology
Bilavčík, Alois ; Faltus, Miloš ; Zámečník, Jiří
In the Czech Republic, ex situ and in vitro conservation is used for apple tree germplasm conservation. In relation to the strategy of maintenance of broad genetic potential of apple tree, there is a necessity to preserve genotypes by storage at ultralow temperatures – cryoconservation. Based on broad spectrum of apple cultivars, an optimised dormant bud cryopreservation protocol was developed. The developed cryoprotocol enables to establish a safe backup storage system for ex situ and in vitro collections of apple tree germplasm. The Ministry of Agriculture of the Czech Republic is the user of this methodology and it will utilize it in the framework of “National Programme on Conservation and Utilization of Plant, Animal and Microbial Genetic Resources for Food and Agriculture”.
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Apple Trees as Representatives the Utility Plants of the Tribe Rosaceae
Šnorová, Markéta ; Skýbová, Jana (advisor) ; Odcházelová, Tereza (referee)
TITLE Apple trees as representatives of utility plants in the menials Rosaceae AUTHOR Markéta Šnorová DEPARTMENT Department of biology and environmental education SUPERVISOR RNDr. Jana Skýbová ABSTRACT Submitted bachelor thesis is devoted to apple trees, as represantatives of menials Rosaceae. At first I introduced some other plants of family Rosaceae. My object was process theme apple tree from wider angle of view. From pollination until salvage and use their fetuses - apples. I have dealt also ecological requirements of appletree, their cultivation, varieties and history of their cultivation. Further I have highlighted importance of apples for humans in terms of nutrition. For the purpose of teaching i have compiled practical works useful in the lessons of botany at elementary school. And grammar school I suggested and laboratories tasks of chemistry and biology. KEY WORDS Apple tree, apple, cultivation, variety, nutrition.
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Diversity of Neofabraea species causing bull's eye rot in the Czech Republic
Pešicová, Kamila ; Novotný, David (advisor) ; Mazáková, Jana (referee)
Neofabraea is a genus of an important plant pathogenic fungi having worldwide appearance. Four Neofabraea species are responsible for bull's eye rot of pome fruits. The aim of this thesis was to investigate which of these species occur in the Czech Republic. 81 isolates were collected during a two- year period and they were identified using PCR fingerprinting (primers ERIC 1R and M13-core) and DNA sequencing (ITS, mtSSU and tub2). The results showed that species N. alba, N. perennans and Cryptosporiopsis kienholzii occur in the Czech Republic. According to available information, this is the second record of C. kienholzii in Europe. One isolate (KP4) failed to be identified as any of the species. KP4 is very close to C. kienholzii, but it can be distinguished both biologically and genetically. Furthermore, the aggressiveness of individual species was compared.N. perennans and strain KP4 proved to be most aggressive, the least aggressive is C. kienholzii. Two N. alba strains (KP36 and KP37) isolated from healthy apple fruit and leaf are pathogenic for apple fruits. Keywords: aggressiveness, Cryptosporiopsis kienholzii, Dermateaceae, Helotiales, apple tree, Malus, PCR fingerprinting, postharvest diseases Powered by TCPDF (www.tcpdf.org)
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The energetic use possibilities of wood from apple tree pruning and some other
Usťak, Sergej ; Šinko, Jan ; Honzík, Roman
The objective of this methodology is to provide to farmers, agricultural consultants, manufacturers of shaped solid biofuels, operators of boilers using biofuels, workers in research and agricultural education, and to all others interested persons in growing and processing agricultural biomass as a renewable source of raw materials and energy, the basic information about the energetic use of wood from apple tree pruning and some other fruit woods. Applying of this methodology in practice can provide environmentally acceptable and economically effective use of such wood waste. At the introduction, this work provides a basic overview of literary information on relevant issues. Furthermore, the methodology describes own outcomes obtained during the research project usable in practice and provides chemical, energetic and economic evaluation. At the conclusion is presented a summary assessment of the achieved outcomes and a general recommendation for practice.
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